Journal: Respiratory Research

Article Title: FK506-binding protein 10 (FKBP10) regulates lung fibroblast migration via collagen VI synthesis

doi: 10.1186/s12931-018-0768-1

Figure Lengend Snippet: Primer table for Real-Time Quantitative Reverse-Transcriptase PCR (qRT-PCR). Primers were synthesized by MWG Eurofins (Ebersberg, Germany).

Article Snippet: Integrin-β1 , ITGB1 , mouse monoclonal anti-ITGB1 antibody , Abcam, Cambridge, UK , WB.

Techniques: Synthesized

Journal: Respiratory Research

Article Title: FK506-binding protein 10 (FKBP10) regulates lung fibroblast migration via collagen VI synthesis

doi: 10.1186/s12931-018-0768-1

Figure Lengend Snippet: Primary antibodies used in Western Blot analysis, Immunofluorescence and Proximity Ligation Assays

Article Snippet: Integrin-β1 , ITGB1 , mouse monoclonal anti-ITGB1 antibody , Abcam, Cambridge, UK , WB.

Techniques: Western Blot, Immunofluorescence, Ligation

Journal: Respiratory Research

Article Title: FK506-binding protein 10 (FKBP10) regulates lung fibroblast migration via collagen VI synthesis

doi: 10.1186/s12931-018-0768-1

Figure Lengend Snippet: FKBP10 deficiency alters the expression of molecules implicated in adhesion and migration. a, c, e, g, i Western Blot analysis of phLF treated with scrambled siRNA as control (sc) or FKBP10 siRNA (kd) and 2- phosphoascorbate (0.1 mM) in absence and presence of TGF-β1 (2 ng/mL) for 48h. Densitometric analysis and representative blots show the effect of FKBP10 kd on the expression of talin-1 (TLN1) ( a ), calpain-4 (CAPNS1) ( c ), integrin β1 (ITGB1) ( e ), caveolin-1 (CAV1) ( g ) and coronin 1C (CORO1C) ( i ) relative to β-actin as loading control (ACTB). b, d, f, h, j Quantitative reverse transcriptase-polymerase chain reaction analysis of phLF treated with scrambled siRNA as control (sc) or FKBP10 siRNA (kd) and 2- phosphoascorbate (0.1 mM) in absence and presence of TGF-β1 (2 ng/mL) for 48h. Transcript levels are presented as -ΔCt values showing the effect of siRNA mediated kd of FKBP10 on talin-1 (TLN1) ( b ), calpain-4 (CAPNS1) ( d ), integrin β1 (ITGB1) ( f ), caveolin-1 (CAV1) ( h ) and coronin 1C (CORO1C) ( j ). DEAH (Asp-Glu-Ala-His) Box Polypeptide 8 (DHX8) was used as endogenous control. All data is based on eight (protein) or seven (mRNA) independent experiments. Statistical significance between control and FKBP10 kd is indicated by horizontal brackets and asterisks

Article Snippet: Integrin-β1 , ITGB1 , mouse monoclonal anti-ITGB1 antibody , Abcam, Cambridge, UK , WB.

Techniques: Expressing, Migration, Western Blot, Polymerase Chain Reaction

Journal: Cell and Tissue Banking

Article Title: Multilineage potential research of Beijing duck amniotic mesenchymal stem cells

doi: 10.1007/s10561-018-9701-6

Figure Lengend Snippet: Primer sequences used for RT-PCR

Article Snippet: The direct antibodies used were FITC goat anti-rabbit CD29 (1:100; BIOSS), FITC goat anti-rabbit CD166 (1:100; BIOSS), FITC goat anti-rabbit CD71 (1:100; BIOSS), FITC goat anti-rabbit CD105 (1:100; BIOSS) and FITC goat anti-rabbit OCT4 (1:100; BIOSS).

Techniques:

Journal: Cell and Tissue Banking

Article Title: Multilineage potential research of Beijing duck amniotic mesenchymal stem cells

doi: 10.1007/s10561-018-9701-6

Figure Lengend Snippet: Detection of surface markers in AMSCs. a The AMSCs could express pluripotent marker gene OCT4 and MSC-associated markers by immunofluorescence stain (bar = 50 μm), DAPI, Blue. b mRNA expression levels of AMSCs markers were detected by RT-PCR, but the expression of CD34 and CD45 were not detected. c AMSCs at P4 were colabeled with surface antigens (CD29, CD166, CD71, CD105, OCT4), and the positive rates were all above 99% by flow cytometry analysis

Article Snippet: The direct antibodies used were FITC goat anti-rabbit CD29 (1:100; BIOSS), FITC goat anti-rabbit CD166 (1:100; BIOSS), FITC goat anti-rabbit CD71 (1:100; BIOSS), FITC goat anti-rabbit CD105 (1:100; BIOSS) and FITC goat anti-rabbit OCT4 (1:100; BIOSS).

Techniques: Marker, Immunofluorescence, Staining, Expressing, Reverse Transcription Polymerase Chain Reaction, Flow Cytometry